Use for: TIRF imaging; Screening; bright field/DIC imaging; fluorescence imaging; fixed and live cell imaging; multi position imaging; imaging of samples on slides, dishes and multi well plates; time series; Z-series.
With funding from the University Equipment Fund this system has been upgraded with Nikon JOBS software and a 20x objective in August 2013. The JOBS software is a module of NIS-Elements which allows us to use this microscope as a screening station, using slides, dishes or multi well plates.
The Nikon TIRF microscope is a fully motorized system build on a Nikon eclipse Ti microscope. This microscope is has a 488 nm and a 568 nm laser for TIRF and a LED light source for the wavelengths 365nm, 490nm, 565nm and 635nm for normal epifluorescence microscopy. For fast live cell imaging a dual filter block for excitation with 490 nm and 565 nm has been installed on this system. This filter block can be used for both LED and TIRF imaging. The system is has an environmental chamber with CO2 and temperature control as well as the Nikon Perfect Focus System (PFS) to keep the samples in focus during long live time imaging experiments.
For image acquisition an Andor iXonEM+ EMCCD DU 885 camera is attached to the microscope. This camera can operate in both EM-CCD and CCD mode, has a pixel size of 8 x 8 µm and an image size of 1004 x 1002 pixels.
Model: TI-E Inverted Microscope