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Maldi TOF 4700 Proteomics Analyser.

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Maldi TOF 4700 Proteomics Analyser. For matrix-assisted laser desorption/ionisation-time of flight mass spectrometry, produced and supplied by Applied Systems and first in service in 2003. MALDI-TOF MS is a relatively novel technique in which a co-precipitate of an UV-light absorbing matrix and a biomolecule is irradiated by a nanosecond laser pulse. Most of the laser energy is absorbed by the matrix, which prevents unwanted fragmentation of the biomolecule. The ionized biomolecules are accelerated in an electric field and enter the flight tube. During the flight in this tube, different molecules are separated according to their mass to charge ratio and reach the detector at different times. In this way each molecule yields a distinct signal. The method is used for detection and characterization of biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides, with molecular masses between 400 and 350,000 Da. It is a very sensitive method, which allows the detection of low (10-15 to 10-18 mole) quantities of sample with an accuracy of 0.1 - 0.01 %. Liaise with primary contact to learn more and discuss access.

Contact SKELTON, Dr Peter pcs22@cam.ac.uk +441223336387

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