Luminescence is excitation of a molecule resulting in fluorescence caused by a chemical reaction, either a biological one, bioluminescence, or a purely chemistry based one, chemiluminescence, or by an external light source resulting in photoluminescence. Normal fluorescence microscopes are an example of photoluminescence. The LV200 makes use of bioluminescence and chemiluminescence. Because of the lack of a light source there is no background or autofluorescence when using luminescence imaging resulting in a high signal-to-noise ratio.
Specifications for the LV200 This system is specifically developed for bioluminescence imaging. A built-in system for temperature control, humidity, and gas flow helps to keep the cultured cells or tissue slices healthy for long time (days) experiments. The system can handle small samples in 35 mm dishes.
LV200 with closed condenser (left) and with upwards turned condenser (right) revealing the culture chamber.
An Hamamatsu C9100-13 EM-CCD camera is used for image acquisition. This allows fast imaging at low light levels (using EM-CCD mode) as well as long exposures times at low light levels (using most likely CCD mode). Pixel size of the camera is 16 x 16 µm with an image size of 512 x 512 pixels.
Objectives available: UPLSAPO 10x NA=0.4 UPLSAPO 20x NA=0.75 UPLFLN 40x Oil NA=1.3 UPLSAPO 60x Oil NA=1.35
The system has a high NA tube lens optimized for luminescence imaging with a 0.2 x magnification, all objectives have therefore an effective magnification of magnification x 0.2 (2x, 4x, 8x and 12x).
Model: Bioluminescence Imaging System LV200