An analytical HPLC system is broadly made up of a liquid mobile phase pump, column, and detector. HPLC is used to separate a sample mixture in solution by separating sample components by their relative affinity for the column as opposed to the mobile phase. Commonly a reverse phase column is used which separates components in order of increasing hydrophobicity. The components then pass through the UV detector which gives a signal proportional to component concentration. The CAD detector can detect analytes that have no chromophore, and has similar application to an ELSD detector. A PC can then be used to integrate the signal for quantification.
Model: Ultimate 3000
Manufacturer: Thermo Scientific