Equipment from

http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx">http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx

The advantages of the NMR method include the ability to obtain information at atomic resolution using aqueous solutions, biological fluids and tissue extracts that resemble physiological conditions. HWBNMR is the UK's largest NMR facility, providing academic and industrial users with open access tosix NMR spectrometers operating at 500-900 MHz, four cryogenic probes and high throughput autosamplers

http://www.birmingham.ac.uk/facilities/nmr/access/index.aspx">http://www.birmingham.ac.uk/facilities/nmr/access/index.aspx

White Light Laser Source and Near Field Optical Microscope Purchased as part of the ERDF funded Creating and Characterisating Next Generation of Advanced Materials Project. Park high resolution AFM, Park Near Field Optical Microscope (NSOM), SC400 Fianium white laser source, Hammamatsu PMT, Andor CCD spectrometer, Tsunami laser. The AFM permits to achieve morphological analysis of the samples with a resolution less than 1. Confocal system permits to achieve 3D optical images of the samples and to analyse their fluorescence properties. White laser source provide broadband excitation of the samples.

Structural/elemental/electronic atomic scale characterisation of nanoscale cluster/materials Purchased as part of the ERDF funded Creating and Characterisating Next Generation of Advanced Materials Project the JEOL Aberration-Corrected Transmission Electron Microscope (JEM-2100F) High resolution imaging, Materials qualification, Chemical analysis, Particles detection/characterisation, Defects analysis, Microstructural characterisation.

Liquid helium supply (liquefing helium gas, recycled from various labs withi the School)

HP8720 microwave network analyser

SPUTTER UNIT

Located on the 8th floor of Biosciences. Laser scanning confocal microscope with integrated fluorescence correlation spectroscopy (FCS)

The Biomark genetic analyser is a high throughput PCR machine allowing traditional TaqMan assays to be carried out on a hitherto unpredcedented large scale. The instrument can be used in a 48:48 or 96:96 array format making it possible to perform 9216 separate PCR reactions on one chip.

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with mm wave system, cal kits

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The LSM780 has 405, 458, 488, 514, 561, 594 and 633 laser lines and is capable of spectral separation of dyes. It can be used for live cell confocal imaging having an inverted heated stage and an incubation chamber. The stage is fully motorised.

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas. For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Fast dynamic analysis of all sorts of materials Purchased as part of the ERDF funded Creating and Characterisating Next Generation of Advanced Materials Project the Ti:Sapphire Femtosecond Laser System

Electron beam looking at nanoscopic features of conductive materials/samples Purchased as part of the ERDF funded Creating and Characterisating Next Generation of Advanced Materials Project the Low Temperature Scanning Tunnelling Microscope can image and probe individual atoms. This allows electronic and vibrational spectroscopy with atomic-scale spatial resolution. They can also image larger areas of surfaces (~ 2 mm2) still with high spatial resolution. Possible applications range from probing the fundamentals of surface induced catalysis to arranging individual atoms in pre-designed structures, e.g., writing words and letters using individual atoms.

http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx">http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx

M2 Cusing Laser machine uses Diode pumped Yb-Fibre laser with 200w effective output. It uses reactive and non-reactive powder materials using Laser cusing Technology. The process module consists of handling station, powder handling and inert gas filtration for flammable or explosive materials. The process chamber is sealed with redundant oxygen online analysis. The build envelope 250x250x280mm (x,y,z). All concept laser powders are 100% reusable for subsequent builds. The powder materials used currently are Aluminium Alloy (GD-AlSi12) and Titanium Alloy (Eli).

PXRD with two heated sample cells (500 & 900C) and gas control system (10 & 100 bar)

http://www.hydrogen.bham.ac.uk">http://www.hydrogen.bham.ac.uk

Particle measuring equipment

High resolution system to measure light spectra/wavelengths. resolution: 0.37nm, wavelength coverage: 250nm-1125nm.

MRS Scanner

MRS Scanner

Positron Emission Tomography

The Dualbeam Strata 235 combined focused ion beam and scanning electron microscope

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http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx">http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx

Examples of the sort of approaches that can be used include: Quantitatively compare protein expression levels of cell lines and tissue samples. Identify and quantitatively compare the constituents of protein complexes and organelles. Identify sites of and quantitatively compare protein/peptide phosphorylation. In order to carry out this work a range of new Bruker mass spectrometers, HPLCs, a full range of peripheral and computer equipment/software are available within The Proteomics Facility in the College of Medical and Dental Sciences. The instruments available are the newly released ultraFlextreme MALDI, the newly released ETD enabled amaZon ion trap and the ultra high resolution ESI tandem MS TOF maXis, all of which are linked to Dionex HPLCs.

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

Examples of the sort of approaches that can be used include: Quantitatively compare protein expression levels of cell lines and tissue samples. Identify and quantitatively compare the constituents of protein complexes and organelles. Identify sites of and quantitatively compare protein/peptide phosphorylation. In order to carry out this work a range of new Bruker mass spectrometers, HPLCs, a full range of peripheral and computer equipment/software are available within The Proteomics Facility in the College of Medical and Dental Sciences. The instruments available are the newly released ultraFlextreme MALDI, the newly released ETD enabled amaZon ion trap and the ultra high resolution ESI tandem MS TOF maXis, all of which are linked to Dionex HPLCs.

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

FluoView FV1000 laser scanning microscope on BX61WI motorized microscope. Confocal part with 4 confocal (2x spectral) detectors + one regular transmitted light detector, laser platform with 4 laser heads featuring 6 laser lines and AOTF laser control.

Analysing images (1 micrometer or less) of various materials e.g. crystal surfaces

Leica EBL100 Kv Nanowriter

The Advanced Mass Spectrometry Facility in the School of Biosciences at the University of Birmingham has extensive experience of this technology for different applications and especially for analysis of complex mixtures such as proteins and metabolites.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

Particle measuring equipment

The Legacy is equipped with three lasers and can detect up to 11 parameters. It is routinely used for high speed sorting with up to 50,000 cells per second, single cell sorting into PCR plates for gene expression analysis or cloning.

Capabilities- 1 Sort 4 selected populations simultaneously 2 Enrich cell populations at rates of up to 80000 cells/sec 3 Puritysorts at rates of up to 30000 cells/sec 4 Sort cells directly into multi-well plates

Under the right conditions purity levels of >99 per cent and recoveries of >95 per cent can be achieved

lasers- 360,488,and 633nm 11 analysis channels- Forward scatter, side scatter, 5 fluorescence channels activated by 488nm laser 2 fluorescence channels activated by 360nm laser 2 fluorescence channels activated by 633nm laser

Suitable fluorophoresinclude FITC, RPE, PE-Cy5, PE-Cy7, PE-Texas Red, Hoechst, APC and APC-Cy7

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

The Advanced Mass Spectrometry Facility in the School of Biosciences at the University of Birmingham has extensive experience of this technology for different applications and especially for analysis of complex mixtures such as proteins and metabolites.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

CUSTOM 75uW DILUTION REFRIGERATOR SYSTEM

Analytical UltraCentrifuge (AUC)

ultracentrifuge

JEM -2100 LaB6 System

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http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx">http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx

The facility has the ability to evaluate many types of electrical energy storage and power converters such as fuel cells, battery packs and DC/DC converters. Furthermore, with peak source and sink powers in excess of 125kW, and typical control loop response times of 10ms, we can enable the dynamic evaluation of a wide range of devices for a wide range of industry and research applications.

Features include:

http://www.birmingham.ac.uk/facilities/esil/equipment/powercycler.aspx">http://www.birmingham.ac.uk/facilities/esil/equipment/powercycler.aspx

The Advanced Mass Spectrometry Facility in the School of Biosciences at the University of Birmingham has extensive experience of this technology for different applications and especially for analysis of complex mixtures such as proteins and metabolites.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

Magnetron sputtering system with 4 targets to produce thin-films

http://www.hydrogen.bham.ac.uk">http://www.hydrogen.bham.ac.uk

http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx">http://www.birmingham.ac.uk/facilities/electron-microscopy/microscopes/index.aspx

STS Multiplex Inductive Coupled Plasma Reactive Ion Etching System

The advantages of the NMR method include the ability to obtain information at atomic resolution using aqueous solutions, biological fluids and tissue extracts that resemble physiological conditions. HWBNMR is the UK's largest NMR facility, providing academic and industrial users with open access tosix NMR spectrometers operating at 500-900 MHz, four cryogenic probes and high throughput autosamplers

http://www.birmingham.ac.uk/facilities/nmr/access/index.aspx">http://www.birmingham.ac.uk/facilities/nmr/access/index.aspx

FAB (Fast Atom Bombarbment) or LSIMS (Liquid Secondary Ion Mass Spectrometry) analysis is normally used for samples that are insufficiently volatile to be analysed by electron impact or chemical ionisation. Samples run by LSIMS tend to be of medium to high mass range, our Zabspec has a mass range of 10,000 Daltons at 8Kv accelerating voltage.

http://www.birmingham.ac.uk/facilities/chemical-analysis/mass-spec/fast-atom-bombardment.aspx">http://www.birmingham.ac.uk/facilities/chemical-analysis/mass-spec/fast-atom-bombardment.aspx

The XDP is equipped with three lasers and can detect up to 11 parameters. It is routinely used for high speed sorting with up to 50,000 cells per second, single cell sorting into PCR plates for gene expression analysis or cloning.

Capabilities- 1 Sort 4 selected populations simultaneously 2 Enrich cell populations at rates of up to 80000 cells/sec 3 Puritysorts at rates of up to 30000 cells/sec 4 Sort cells directly into multi-well plates

Under the right conditions purity levels of >99 per cent and recoveries of >95 per cent can be achieved

lasers- 360,488,and 633nm 11 analysis channels- Forward scatter, side scatter, 5 fluorescence channels activated by 488nm laser 2 fluorescence channels activated by 360nm laser 2 fluorescence channels activated by 633nm laser

Suitable fluorophoresinclude FITC, RPE, PE-Cy5, PE-Cy7, PE-Texas Red, Hoechst, APC and APC-Cy7

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

The FACSAria II is equipped with:Three air-cooled lasers at 488 nm, 633 nm and 375 nm; multicolour analysis up to 10 parameters. A variety of sterile sorting devices are available from bulk sorting into tubes, multi-well formats and slides and plates.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

Comet T-Scan Triangulation Scanner

Atomic force microscope capable of imaging in air and fluid, as well as making electrical measurements. Large scope of samples may be studied such as nanoparticles, electrode surfaces, thin films and biological samples.

http://www.birmingham.ac.uk/facilities/advanced-materials-characterisation-2/facilities/index.aspx">http://www.birmingham.ac.uk/facilities/advanced-materials-characterisation-2/facilities/index.aspx

NMR Spectroscopy is now an extremely powerful technique for solving many chemical problems. The availability of high-field magnets has dramatically improved the sensitivity and signal disposition, and improved electronics and instrument design have made it possible to observe previously 'exotic' nuclei.

The Bruker DRX500 features:

http://www.birmingham.ac.uk/facilities/chemical-analysis/nmr-spectroscopy.aspx">http://www.birmingham.ac.uk/facilities/chemical-analysis/nmr-spectroscopy.aspx

Ti-E Motorised inverted microscope system

Total system; parts

Raman with two heated sample cells (500 & 600C) and gas control system (1 & 100 bar)

http://www.hydrogen.bham.ac.uk">http://www.hydrogen.bham.ac.uk

Carry out protein identification and function studies. Study protein interactions. Identify potential drug targets and diagnostic markers

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

DNA Sequencing and Fragment analysis. The DNA Sequencing "Plasmid to Profile" service runs on Tuesday and Friday for small numbers of samples, or any day for full plates. Simply supply DNA and primers. Self load samples or send by post. The service utilises ABI Big Bye Terminator chemistry and Automated liquid handling ensuring consistency and accuracy. Samples are analysed on the ABI 3730 and resolved on 50cm capillaries. Data is available online by 9pm the same day for plate 1 and at four hourly intervals for subsequent plates. An online trouble shooting guide is available as well as in house customer support. Software for viewing data available as download from website. Fragment analysis by arrangement, running of pre-prepared samples on a run only basis.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

Nikon A1R Inverted Confocal/TIRF on 8th floor of Biosciences with resonant scanner, environmental chamber, spectral detector, EMCCD, 'Perfect Focus System' and motorised XYZ (including piezo-Z)

Included in the diverse applications of this technology are: targeted re-sequencing (including exome), whole genome re-sequencing, small/microRNA sequencing, transcriptomes (RNA-Seq; gene expression and alternative/novel isoforms discovery), biomarker discovery; gene-fusions/chromosome re-arrangements, SNP/mutation detection, chromatin immunoprecipitation (ChIP-Seq analyses), SAGE digital gene expression, methylation analyses.

http://mymds.bham.ac.uk/TECHNOLOGYHUB/">http://mymds.bham.ac.uk/TECHNOLOGYHUB/

Particle equipment with 400mJ/532mm laser

Activation of materials to produce a wide range of radioisoptopes

Deposition of size selected metalic clusters for various applications Purchased as part of the ERDF funded Creating and Characterisating Next Generation of Advanced Materials Project the Size Selected Cluster Beam can explore size-dependent physical and chemical properties of nanoparticles. The technique is inspired by the expectation that due to nanoparticles reduced dimensionality novel properties can arise that are not present in the bulk counterparts. The state-of-the-art high vacuum system consists of a magnetron sputtering gas condensation cluster beam source and a unique lateral time-of-flight mass filter. This system allows synthesis of size-selected nanoparticles up to 75000 atoms with a mass resolution of M/?M ? 130. The size-selected cluster beam can be deposited onto many support materials at various impact (kinetic) energies and pinned by surface defects

The Advanced Mass Spectrometry Facility in the School of Biosciences at the University of Birmingham has extensive experience of this technology for different applications and especially for analysis of complex mixtures such as proteins and metabolites.

http://www.genomics.bham.ac.uk/default.htm">http://www.genomics.bham.ac.uk/default.htm

Bioprocessing pilot plant facilities 2 x 100L Fermenters ( Pierre Guerin.) Bought with Advantage West Midlands Biohydrogen funding in 2007 these two state-of-the-art large scale stainless steel vessels are fully automated with sterilisation and fermentation cycles and are fully computer controlled. Set in parallel to each other, they can be operated entirely independently. They are designed for microbial fermentation and hydrogen production, and can be used to grow organisms at ACDP and ACGM class 1. They can be used to grow aerobic and anaerobic cultures, sparging with air (via a compressor) or nitrogen (via a nitrogen generator) in batch and fed-batch modes. Aeration is regulated by a cascade control (airflow / agitator speed) via a DOT probe. pH and antifoam control loops are also present. Sensor and process data are logged on the control computer. Online off-gas analysis is possible with a Thermo Fisher Prima db mass spectrometer. Biomass can be harvested using a Carr Centritech powerfuge (BioProcess Engineering Services Ltd). Cells can be disrupted using a Constant Systems pilot-scale disruptor.

Quantum Laser Machine uses Rofin CO2 laser of 1750 w TRIAGON SERIES. The wavlength is 10.6microns, continuous wave, beam diameter of 14mils. It can be used for various applications of powder deposition in 3 axial movements, using coaxial nozzle or with coaxial wire feed.It has the capability of welding, cutting and peripheral micromachining applications of the Laser.

The Leica LSI TCS zoom confocal can obtain high quality images from cells to specimens with a 16mm field of view. Illumination can be brightfield with a black and white or colour mode camera and/or confocal with 405, 488, 532 and 635 laser excitation. Various modes of zoom are possible with 2x 0.2NA or 5x 0.5 NA objectives. Specimen holders include plate and slide holders and a rotatable dish holder.

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Trumpf Lasercell 1005 is a high volume CNC Laser cutting, welding, deposition or surface treatment machine. It has flying optics with 6.5 acis movemnet hence used for making complex geometries in deposition of the parts. It is 4Kw Trudisk laser with Fiber optic laser delivery to the substrate.

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